Rabbit Anti-Human CCT3 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P49368 |
| Gene Id | 7203 |
| Gene Symbols | CCT3 |
| Protein Name / Synonyms | CCT3 (Center) |
| Clonality | Polyclonal |
| Preparation | This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the Central region of human CCT3. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Monkey, Xenopus
Other Names
CCT3; CCTG; TRIC5; T-complex protein 1 subunit gamma; CCT-gamma; hTRiC5
NCBI Accession #
NP_001008800.1;NP_005989.3
Other Accession #
P50143; Q6P502; P80318; Q4R963; Q3T0K2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
60534
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of CCT3 Antibody (Center) (Cat# 102-15208) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab. |
 | Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) (Cat# 102-15208). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) . |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.