Rabbit Anti-Human CD1E (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P15812 |
| Gene Id | 913 |
| Gene Symbols | CD1E |
| Protein Name / Synonyms | CD1E (Center) |
| Clonality | Polyclonal |
| Preparation | This CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human CD1E. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.
Specificity
Other Names
CD1E; T-cell surface glycoprotein CD1e, membrane-associated; R2G1; CD_antigen=CD1e; T-cell surface glycoprotein CD1e, soluble
NCBI Accession #
NP_001036048.1;NP_001036049.1;NP_001036050.1;NP_001036051.1;NP_001036052.1;NP_001172036.1;NP_001172037.1;NP_001172039.1;NP_001172041.1;NP_001172042.1;NP_001172043.1;NP_001172044.1;NP_112155.2
Other Accession #
NP_112155.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43626
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | CD1E Antibody (Center) (Cat# 102-18452) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow). |
 | CD1E Antibody (Center) (Cat# 102-18452) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | CD1E Antibody (Center) (Cat# 102-18452) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of CD1E Antibody (Center)(Cat# 102-18452) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.