Synonyms
BTLA1 antibody, CD272 antibody, FLJ16065 antibody, MGC129743 antibody, BTLA antibody, B- and T-lymphocyte-associated protein antibody, B- and T-lymphocyte attenuator antibody, B and T lymphocyte attenuator antibody, B and T lymphocyte associated antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of CD272
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

CD272 antibody detects BTLA protein by Western blot analysis.
A. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
CD272 antibody (107-11038) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CD272(107-11038) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.