CD44 antibody, 0.89 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 960 |
| Protein Name / Synonyms | CD44 |
| Concentration (lot specific) | 0.89 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MGC10468 antibody, MIC4 antibody, Pgp1 antibody, CD44 antibody, heparan sulfate proteoglycan antibody, GP90 lymphocyte homing/adhesion receptor antibody, phagocytic glycoprotein 1 antibody, homing function and Indian blood group system antibody, cell surface glycoprotein CD44 antibody, extracellular matrix receptor III antibody, CD44 antigen antibody, Hermes antigen antibody, chondroitin sulfate proteoglycan 8 antibody, epican antibody, hematopoietic cell E- and L-selectin ligand antibody, hyaluronate receptor antibody, CD44 molecule (Indian blood group) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
82 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 258 of CD44
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44(107-10319) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: MCF-7
10% SDS PAGE
107-10319 diluted at 1:10000
A: A549
B: H1299
C: MCF-7
10% SDS PAGE
107-10319 diluted at 1:10000
CD44 antibody detects CD44 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
7.5 % SDS-PAGE
CD44 antibody (107-10319) dilution: 1:5000
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
7.5 % SDS-PAGE
CD44 antibody (107-10319) dilution: 1:5000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using CD44 (107-10319) antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(107-10319) antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(107-10319) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.