ELISA (recommended work dilution= 1:160,000 (at least detecting 6.25 µg/ml))
Immunogen was synthetic peptide derived from Human CD79alpha . This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
CD79 (Cluster of Differentiation 79) is a transmembrane protein that forms a complex with the B-cell receptor (BCR) and generates a signal following recognition of antigen by the BCR. CD79 is composed of two distinct chains called CD79A and CD79B (formerly known as Ig-alpha and Ig-beta); these form a heterodimer on the surface of a B cell stabilized by disulfide bonding. CD79alpha and CD79b are both members of the immunoglobulin superfamily. Human CD79alpha is encoded by the mb-1 gene that is located on chromosome 19, and CD79b is encoded by the B29 gene that located on chromosome 17.Both CD79 chains contain an immunoreceptor tyrosine-based activation motif (ITAM) in their intracellular tails that they use to propagate a signal in a B cell, in a similar manner to CD3 generated signal tranduction observed during T cell receptor activation on T cells.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat CD79alpha has not yet been tested.
Mouse Anti-Human CD79alpha (cat#DS-MB-03199)Mouse Anti-Human CD79alpha, FITC-labeled(cat#DS-MB-03200)
- Li S, Borowitz MJ. CD79a(+) T-cell lymphoblastic lymphoma with coexisting Langerhans cell histiocytosis. Arch Pathol Lab Med. 2001 Jul;125(7):958-60.
- Chu PG, Arber DA. CD79: a review. Appl Immunohistochem Mol Morphol. 2001 Jun;9(2):97-106.
12 months from the date of shipment when stored properly.