CDCA1 antibody, 0.35 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 83540 |
| Protein Name / Synonyms | CDCA1 |
| Concentration (lot specific) | 0.35 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CDCA1 antibody, CT106 antibody, NUF2R antibody, NUF2 antibody, cell division cycle-associated protein 1 antibody, hNuf2R antibody, hsNuf2 antibody, kinetochore protein Nuf2 antibody, cell division cycle associated 1 antibody, cancer/testis antigen 106 antibody, hNuf2 antibody, "NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
54 kDa
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1(107-10934) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
10% SDS PAGE
107-10934 diluted at 1:5000
A: HeLa
B: HeLa nucleus
10% SDS PAGE
107-10934 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.