CHMP5 antibody [N1C3], 0.7 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 51510 |
| Protein Name / Synonyms | CHMP5 |
| Concentration (lot specific) | 0.7 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C9orf83 antibody, HSPC177 antibody, PNAS-2 antibody, SNF7DC2 antibody, Vps60 antibody, CHMP5 antibody, SNF7 domain containing 2 antibody, SNF7 domain-containing protein 2 antibody, chromatin-modifying protein 5 antibody, chromatin modifying protein 5 antibody, vacuolar protein sorting-associated protein 60 antibody, apoptosis-related protein PNAS-2 antibody, hVps60 antibody, charged multivesicular body protein 5 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
25 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 202 of CHMP5 (Uniprot ID# Q9NZZ3)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
107-10639 diluted at 1:1000
A: mouse stomach
12% SDS PAGE
107-10639 diluted at 1:1000
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis.
Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (107-10639) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP5 protein stained by CHMP5 antibody [N1C3] (107-10639) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5(107-10639) antibody at 1:100 dilution.
Sample(30µg whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
12% SDS PAGE
107-10639 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
12% SDS PAGE
107-10639 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.