Rabbit Anti-Human CKM (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06732 |
| Gene Id | 1158 |
| Gene Symbols | CKM |
| Protein Name / Synonyms | CKM (C-term) |
| Clonality | Polyclonal |
| Preparation | This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Specificity
Predicted Reactivity
Bovine, Pig, Rabbit
Other Names
CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK
NCBI Accession #
NP_001815.2
Other Accession #
P00563; Q5XLD3; Q9XSC6
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43101
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | The anti-CKM Pab (Cat# 102-17113) is used in Western blot to detect CKM in C6 cell lysate. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.