Synonyms
CLIC3 antibody, chloride intracellular channel protein 3 antibody, chloride intracellular channel 3 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
27 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 236 of CLIC3 (Uniprot ID# O95833)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded T(IV)(lung CA), using CLIC3(107-10450) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CLIC3(107-10450) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:HeLa S3
12% SDS PAGE
107-10450 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.