Coronin 1B antibody [N1N3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 57175 |
| Protein Name / Synonyms | Coronin 1B |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CORONIN-2 antibody, DKFZp762I166 antibody, CORO1B antibody, coronin-1B antibody, "coronin, actin binding protein, 1B antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
54 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 213 of Coronin 1B (Uniprot ID# Q9BR76)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10651 diluted at 1:1000
A: NIH-3T3
10% SDS PAGE
107-10651 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using Coronin-1B(107-10651) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
107-10651 diluted at 1:1000
A:293T
10% SDS PAGE
107-10651 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.