COX6B1 antibody [N2C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 1340 |
| Protein Name / Synonyms | COX6B1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
COX6B antibody, COXG antibody, COXVIb1 antibody, COX6B1 antibody, cytochrome c oxidase subunit 6B1 antibody, COX VIb-1 antibody, cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
10 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 23 and 86 of COX6B1 (Uniprot ID# P14854)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
COX6B1 antibody [N2C3] detects COX6B1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COX6B1 protein stained by COX6B1 antibody [N2C3] (107-10745) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COX6B1 protein stained by COX6B1 antibody [N2C3] (107-10745) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1(107-10745) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
15% SDS PAGE
COX6B1 antibody
107-10745 diluted at 1:1000
A: Hep G2
15% SDS PAGE
COX6B1 antibody
107-10745 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.