Rabbit Anti-Human CREB1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P16220 |
| Gene Id | 1385 |
| Gene Symbols | CREB1 |
| Protein Name / Synonyms | CREB1 (Center) |
| Clonality | Polyclonal |
| Preparation | This CREB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-132 amino acids from the Central region of human CREB1. |
| Concentration (lot specific) | 0.252 mg/ml |
| Recommended Applications | FC, Western Blotting, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
CREB1; Cyclic AMP-responsive element-binding protein 1
NCBI Accession #
NP_004370.1;NP_604391.1
Other Accession #
P15337; Q01147; P27925; NP_004370.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
36688
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent image of A549 cell stained with CREB1 Antibody (Center)(Cat# 102-11218).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CREB1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).CREB1 immunoreactivity is localized to Nucleus significantly. |
 | CREB1 Antibody (Center) (Cat# 102-11218) western blot analysis in HT29 cell line lysates (35ug/lane).This demonstrates the CREB1 antibody detected the CREB1 protein (arrow). |
 | CREB1 Antibody (Center) (Cat# 102-11218) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.