Rabbit Anti-Human CUL5 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q93034 |
| Gene Id | 8065 |
| Gene Symbols | CUL5 |
| Protein Name / Synonyms | CUL5 (C-term) |
| Clonality | Polyclonal |
| Preparation | This CUL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 747-774 amino acids from the C-terminal region of human CUL5. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
Specificity
Predicted Reactivity
Rabbit
Other Names
CUL5; VACM1; Cullin-5; Vasopressin-activated calcium-mobilizing receptor 1
NCBI Accession #
NP_003469.2
Other Accession #
Q29425
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
90955
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
 | Western blot analysis of anti-CUL5 Antibody (C-term)(Cat# 102-17427) in Hela cell line lysates (35ug/lane). CUL5 (arrow) was detected using the purified Pab. |
 | Flow cytometric analysis of NCI-H292 cells using CUL5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | CUL5 Antibody (C-term) (Cat# 102-17427) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUL5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.