Synonyms
CYCT2 antibody, FLJ13583 antibody, FLJ90560 antibody, MGC134840 antibody, CCNT2 antibody, SDS-stable vimentin-bound DNA fragment HEF42VIM22 antibody, cyclin-T2 antibody, subunit of positive elongation transcription factor b antibody, cyclin T2b antibody, cyclin T2a antibody, cyclin T2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
81 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 497 and 730 of cyclin T2 (Uniprot ID# O60583)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using cyclin T2(107-10998) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2(107-10998) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10998 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.