Rabbit Anti-Human CYP20A1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q6UW02 |
| Gene Id | 57404 |
| Gene Symbols | CYP20A1 |
| Protein Name / Synonyms | CYP20A1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This CYP20A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human CYP20A1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CYP20A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen.
Specificity
Other Names
CYP20A1; Cytochrome P450 20A1
NCBI Accession #
NP_803882.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52432
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of CYP20A1(arrow) using rabbit polyclonal CYP20A1 Antibody (C-term)(Cat# 102-17358). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CYP20A1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma with CYP20A1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of NCI-H292 cells using CYP20A1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.