Cytokeratin 13 antibody, 0.59 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3860 |
| Protein Name / Synonyms | Cytokeratin 13 |
| Concentration (lot specific) | 0.59 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CK13 antibody, K13 antibody, MGC161462 antibody, MGC3781 antibody, KRT13 antibody, cytokeratin 13 antibody, keratin-13 antibody, keratin, type I cytoskeletal 13 antibody, CK-13 antibody, cytokeratin-13 antibody, keratin 13 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 198 and 438 of Cytokeratin 13
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 13(107-10876) antibody at 1:500 dilution.
Cytokeratin 13 antibody detects KRT13 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
10 % SDS-PAGE
Cytokeratin 13 antibody (107-10876) dilution: 1:10000
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
10 % SDS-PAGE
Cytokeratin 13 antibody (107-10876) dilution: 1:10000
Cytokeratin 13 antibody detects Cytokeratin 13 protein at cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 13 antibody (107-10876) dilution: 1:500.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 13 antibody (107-10876) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 13(107-10876) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.