Cytokeratin 2 antibody [N2C2], Internal, 0.67 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3849 |
| Protein Name / Synonyms | Cytokeratin 2, Internal |
| Concentration (lot specific) | 0.67 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CK-2e antibody, K2e antibody, KRT2A antibody, KRT2E antibody, KRTE antibody, MGC116967 antibody, MGC116968 antibody, KRT2 antibody, epidermal ichthyosis bullosa of Siemens antibody, epithelial keratin-2e antibody, keratin-2 epidermis antibody, cytokeratin-2e antibody, "keratin, type II cytoskeletal 2 epidermal antibody", keratin-2e antibody, type-II keratin Kb2 antibody, keratin 2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2 (Uniprot ID# P35908)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2(107-10350) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2(107-10350) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
A: A431
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.