Synonyms
HA4 antibody, Ha-4 antibody, KRTHA4 antibody, hHa4 antibody, KRT34 antibody, "hard keratin, type I, 4 antibody", "hair keratin, type I Ha4 antibody", "keratin, hair, acidic, 4 antibody", keratin-34 antibody, K34 antibody, "keratin, type I cuticular Ha4 antibody", keratin 34 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
49 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 303 of Cytokeratin 34 (Uniprot ID# O76011)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 34(107-11019) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-11019 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.