Rabbit Anti-Human DCI (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42126 |
| Gene Id | 1632 |
| Gene Symbols | ECI1 |
| Protein Name / Synonyms | DCI (C-term) |
| Clonality | Polyclonal |
| Preparation | This DCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-302 amino acids from the C-terminal region of human DCI. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
DCI is a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.
Specificity
Other Names
ECI1; DCI; Enoyl-CoA delta isomerase 1, mitochondrial; 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase
NCBI Accession #
NP_001171500.1;NP_001910.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
32816
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | DCI Antibody (C-term) (Cat# 102-18020) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the DCI antibody detected the DCI protein (arrow). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with DCI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | DCI Antibody (C-term) (Cat# 102-18020) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.