Synonyms
MGC117410 antibody, MGC138632 antibody, DCK antibody, deoxycytidine kinase antibody, deoxynucleoside kinase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 260 of DCK (Uniprot ID# P27707)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DCK antibody detects DCK protein at cytosol and nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: DCK antibody (107-10695) dilution: 1:250.

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10695 diluted at 1:5000

DCK antibody detects DCK protein by western blot analysis.
A. 50 µg mouse brain extract
12 % SDS-PAGE
DCK antibody (107-10695) dilution: 1:1000

DCK antibody detects DCK protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
B. 30 µg Rat2 whole cell extract
12 % SDS-PAGE
DCK antibody (107-10695) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Deoxycytidine kinase(107-10695) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.