DECR1 antibody [N1C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1666 |
| Protein Name / Synonyms | DECR1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DECR antibody, NADPH antibody, SDR18C1 antibody, DECR1 antibody, "short chain dehydrogenase/reductase family 18C, member 1 antibody", "2,4-dienoyl-CoA reductase, mitochondrial antibody", 4-enoyl-CoA reductase antibody, "2,4-dienoyl CoA reductase 1, mitochondrial antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 335 of DECR1 (Uniprot ID# Q16698)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DECR1 antibody [N1C3] detects DECR1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DECR1 protein stained by DECR1 antibody [N1C3] (107-10856) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DECR1 protein stained by DECR1 antibody [N1C3] (107-10856) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1(107-10856) antibody at 1:500 dilution.
Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10856 diluted at 1:10000
A: mouse liver
10% SDS PAGE
107-10856 diluted at 1:10000
Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
107-10856 diluted at 1:10000
A: Hep G2
10% SDS PAGE
107-10856 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.