Rabbit Anti-Human DFFB (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O76075 |
| Gene Id | 1677 |
| Gene Symbols | DFFB |
| Protein Name / Synonyms | DFFB (N-term) |
| Clonality | Polyclonal |
| Preparation | This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Specificity
Other Names
DFFB; CAD; DFF2; DFF40; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated nuclease; DNA fragmentation factor 40 kDa subunit
NCBI Accession #
NP_004393.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
39110
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of DFFB Antibody (N-term) (Cat# 102-18414) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab. |
 | DFFB Antibody (N-term) (Cat# 102-18414) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | DFFB Antibody (N-term) (Cat# 102-18414) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.