Rabbit Anti-Human DGKZ (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13574 |
| Gene Id | 8525 |
| Gene Symbols | DGKZ |
| Protein Name / Synonyms | DGKZ (C-term) |
| Clonality | Polyclonal |
| Preparation | This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.
Specificity
Other Names
DGKZ; DAGK6; Diacylglycerol kinase zeta; Diglyceride kinase zeta
NCBI Accession #
NP_001099010.1;NP_001186195.1;NP_001186196.1;NP_001186197.1;NP_003637.2;NP_963290.1;NP_963291.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
124128
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | DGKZ Antibody (D1004) (Cat# 102-17779) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow). |
 | Western blot analysis of DGKZ (arrow) using DGKZ Antibody (C-term) (Cat# 102-17779). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.