Rabbit Anti-Human Dnmt3a Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9Y6K1 |
| Gene Id | 1788 |
| Gene Symbols | DNMT3A |
| Protein Name / Synonyms | Dnmt3a |
| Clonality | Polyclonal |
| Preparation | This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken
Other Names
DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase HsaIIIA
NCBI Accession #
NP_072046.2;NP_715640.2;NP_783328.1;NP_783329.1
Other Accession #
Q1LZ53; O88508; Q4W5Z4
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
101858
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-Dnmt3a Pab (Cat# 102-10623) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Lysates from thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01;3(10):553-561) |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.