Rabbit Anti-Human DOK1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q99704 |
| Gene Id | 1796 |
| Gene Symbols | DOK1 |
| Protein Name / Synonyms | DOK1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-39 amino acids from the N-terminal region of human DOK1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
DOK1; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62
NCBI Accession #
NP_001184189.1;NP_001372.1
Other Accession #
Q4QQV2; P97465; Q5EA84
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52392
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-DOK1 Pab (Cat# 102-17563) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term) (Cat# 102-17563). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.