Synonyms
DBM antibody, DBH antibody, dopamine beta-hydroxylase antibody, dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
69 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 8 and 320 of Dopamine beta Hydroxylase (Uniprot ID# P09172)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.

Dopamine beta hydroxylase antibody detects DBH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Dopamine beta hydroxylase antibody (107-10273) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dopamine beta-Hydroxylase(107-10273) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10273 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.