DPP3 antibody [N2C1], Internal, 0.49 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10072 |
| Protein Name / Synonyms | DPP3, Internal |
| Concentration (lot specific) | 0.49 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DPPIII antibody, FLJ11387 antibody, FLJ22331 antibody, DPP3 antibody, dipeptidylpeptidase III antibody, dipeptidyl peptidase 3 antibody, dipeptidyl arylamidase III antibody, dipeptidyl peptidase III antibody, dipeptidylpeptidase 3 antibody, DPP III antibody, dipeptidyl aminopeptidase III antibody, dipeptidyl-peptidase 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 138 and 465 of Dipeptidyl-peptidase 3 (Uniprot ID# Q9NY33)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DPP3 antibody [N2C1], Internal detects DPP3 protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
DPP3 antibody [N2C1], Internal (107-10573) dilution: 1:1000
A. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
DPP3 antibody [N2C1], Internal (107-10573) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dipeptidyl-peptidase 3(107-10573) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using DPP3 (107-10573) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:MOLT4
10% SDS PAGE
107-10573 diluted at 1:1000
A:MOLT4
10% SDS PAGE
107-10573 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.