Synonyms
VHR antibody, DUSP3 antibody, vaccinia H1-related phosphatase antibody, serine/threonine specific protein phosphatase antibody, dual specificity protein phosphatase 3 antibody, dual specificity protein phosphatase VHR antibody, vaccinia virus phosphatase VH1-related antibody, dual specificity phosphatase 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
20 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 185 of DUSP3 (Uniprot ID# P51452)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3(107-10819) antibody at 1:500 dilution.

DUSP3 antibody detects DUSP3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15 % SDS-PAGE, and blotted with DUSP3 antibody (107-10819) diluted by 1:1000

Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
107-10819 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.