Synonyms
DNCI2 antibody, FLJ21089 antibody, FLJ90842 antibody, IC2 antibody, MGC104199 antibody, MGC111094 antibody, MGC9324 antibody, DYNC1I2 antibody, cytoplasmic dynein intermediate chain 2 antibody, "dynein, cytoplasmic, intermediate polypeptide 2 antibody", DH IC-2 antibody, "dynein intermediate chain 2, cytosolic antibody", cytoplasmic dynein 1 intermediate chain 2 antibody, "dynein, cytoplasmic 1, intermediate chain 2 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
71 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 129 and 366 (Q13409) of DYNC1I2
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using DYNC1I2(107-11024) antibody at 1:200 dilution.

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DYNC1I2 antibody (107-11024) dilution: 1:500.

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DYNC1I2 antibody (107-11024) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-11024 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.