Edc3 antibody [N2C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 80153 |
| Protein Name / Synonyms | Edc3, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ21128 antibody, FLJ31777 antibody, LSM16 antibody, YJDC antibody, YJEFN2 antibody, EDC3 antibody, yjeF N-terminal domain-containing protein 2 antibody, hYjeF_N2 antibody, yjeF domain-containing protein 1 antibody, yjeF_N2 antibody, yjeF domain containing antibody, LSM16 homolog (EDC3, S. cerevisiae) antibody, hYjeF_N2-15q23 antibody, enhancer of mRNA-decapping protein 3 antibody, enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
56 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 127 and 376 of Edc3 (Uniprot ID# Q96F86)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Edc3(107-11172) antibody at 1:250 dilution.
Edc3 antibody [N2C2], Internal detects Edc3 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Edc3 antibody [N2C2], Internal (107-11172) dilution: 1:500.
Sample: Paraffin-embedded mouse testis.
Edc3 antibody [N2C2], Internal (107-11172) dilution: 1:500.
Edc3 antibody [N2C2], Internal detects EDC3 protein by Western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Edc3 antibody [N2C2], Internal (107-11172) dilution: 1:1000
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Edc3 antibody [N2C2], Internal (107-11172) dilution: 1:1000
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-11172 diluted at 1:3000
A: A431
7.5% SDS PAGE
107-11172 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.