EIF2B beta antibody [N2C3], 0.43 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8892 |
| Protein Name / Synonyms | EIF2B beta |
| Concentration (lot specific) | 0.43 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
EIF-2Bbeta antibody, EIF2B antibody, EIF2B2 antibody, S20I15 antibody, translation initiation factor eIF-2B subunit beta antibody, eIF-2B GDP-GTP exchange factor subunit beta antibody, S20III15 antibody, "eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
39 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 88 and 323 of EIF2B beta (Uniprot ID# P49770)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using EIF2B beta(107-11080) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-11080 diluted at 1:1000
A: A431
10% SDS PAGE
107-11080 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.