Rabbit Anti-Human EIF4E (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06730 |
| Gene Id | 1977 |
| Gene Symbols | EIF4E |
| Protein Name / Synonyms | EIF4E (N-term) |
| Clonality | Polyclonal |
| Preparation | This EIF4E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Rabbit, Xenopus
Other Names
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
NCBI Accession #
NP_001124150.1;NP_001124151.1;NP_001959.1
Other Accession #
P48597; P63074; P29338; P63073; Q9N0T5; Q9DFS6
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
25097
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-EIF4E Antibody (N-term) Pab (102-14316) in HL60 cell line lysates. EIF4E Antibody (N-term)(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term)(Cat# 102-14316), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.