ELISA (recommended work dilution= 1:160,000 (at least detecting 6.25ng/ml))
Immunogen was synthetic peptide derived from the near C-terminus of human Endoglin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles.
Endoglin. also known as CD105, is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex. It is involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling, endoglin has other functions, such as affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development.
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat Endoglin has not yet been tested.
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Human Endoglin ELISA Kit (Cat# ELH-Endoglin-001 ) Rabbit Anti-Endoglin antibody-N-terminus (Cat# 130-10018)Recombinant Human Endoglin (Cat# 228-10387)
- ten Dijke P, Goumans MJ, Pardali E. Endoglin in angiogenesis and vascular diseases. Angiogenesis. 2008;11(1):79-89. Dallas NA, Samuel S, Xia L, Fan F, Gray MJ, Lim SJ, Ellis LM. Endoglin (CD105): a marker of tumor vasculature and potential target for therapy. Clin Cancer Res. 2008 1;14(7):1931-7.