Rabbit Anti-Human ESR1 isoform1(C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P03372 |
| Gene Id | 2099 |
| Gene Symbols | ESR1 |
| Protein Name / Synonyms | ESR1 isoform1(C-term) |
| Clonality | Polyclonal |
| Preparation | This ESR1 isoform1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-480 amino acids from the C-terminal region of human ESR1 isoform1. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
Specificity
Predicted Reactivity
Mouse, Rat, Pig
Other Names
ESR1; ESR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
NCBI Accession #
NP_000116.2;NP_001116212.1;NP_001116213.1;NP_001116214.1
Other Accession #
P06211; Q29040; P19785; NP_000116.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
66216
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of ESR1 isoform1 Antibody (C-term) Pab (Cat# 102-14353) pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. ESR1 isoform1 Antibody (C-term)(arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform1 Antibody (C-term)(Cat# 102-14353). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ESR1 isoform1 immunoreactivity is localized to cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.