Rabbit Anti-Human ESRRA (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P11474 |
| Gene Id | 2101 |
| Gene Symbols | ESRRA |
| Protein Name / Synonyms | ESRRA (Center) |
| Clonality | Polyclonal |
| Preparation | This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human ESRRA. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ESRRA; ERR1; ESRL1; NR3B1; Steroid hormone receptor ERR1; Estrogen receptor-like 1; Estrogen-related receptor alpha; Nuclear receptor subfamily 3 group B member 1
NCBI Accession #
NP_001269379.1;NP_001269380.1;NP_004442.3
Other Accession #
P62510; P62509; P62508; P11475; Q61539; O95718; Q5QJV7; O08580
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
45510
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of ESRRA Antibody (Center) (Cat# 102-17958) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of ESRRA (arrow) using rabbit polyclonal ESRRA Antibody (Center) (Cat# 102-17958). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ESRRA gene. |
 | Confocal immunofluorescent analysis of ESRRA Antibody (Center) (Cat# 102-17958) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.