Rabbit Anti-Human EWSR1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01844 |
| Gene Id | 2130 |
| Gene Symbols | EWSR1 |
| Protein Name / Synonyms | EWSR1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, FC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EWSR1 is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Specificity
Predicted Reactivity
Mouse
Other Names
EWSR1; EWS; RNA-binding protein EWS; EWS oncogene; Ewing sarcoma breakpoint region 1 protein
NCBI Accession #
NP_001156757.1;NP_001156758.1;NP_001156759.1;NP_005234.1;NP_053733.2
Other Accession #
Q61545
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
68478
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent confocal image of U251 cell stained with EWSR1 Antibody (C-term)(Cat# 102-18046).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly. |
 | Western blot analysis of EWSR1 Antibody (C-term) (Cat# 102-18046) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab. |
 | EWSR1 Antibody (C-term) (Cat# 102-18046) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.