Factor X antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 2159 |
| Protein Name / Synonyms | Factor X |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FX antibody, FXA antibody, F10 antibody, Stuart-Prower factor antibody, prothrombinase antibody, coagulation factor X antibody, factor Xa antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
55 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 33 and 312 of Factor X (Uniprot ID# P00742)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Factor X(107-10870) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Factor X(107-10870) antibody at 1:500 dilution.
Factor X antibody detects F10 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells
10 % SDS-PAGE
Factor X antibody (107-10870) dilution: 1:5000
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human F10-transfected 293T cells
10 % SDS-PAGE
Factor X antibody (107-10870) dilution: 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.