Synonyms
CMT4H antibody, DKFZp313E1818 antibody, DKFZp434K1572 antibody, FLJ34370 antibody, FLJ42663 antibody, FRABP antibody, MGC57222 antibody, ZFYVE6 antibody, FGD4 antibody, "FYVE, RhoGEF and PH domain-containing protein 4 antibody", FGD1-related F-actin-binding protein antibody, "FGD1 family, member 4 antibody", actin-filament binding protein frabin antibody, actin filament-binding protein frabin antibody, zinc finger FYVE domain-containing protein 6 antibody, "FYVE, RhoGEF and PH domain containing 4 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
87 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 49 and 327 of FGD4 (Uniprot ID# Q96M96)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGD4(107-10873) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SG xenograft, using FGD4(107-10873) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10873 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.