FGFR-5 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 53834 |
| Protein Name / Synonyms | FGFR-5 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FGFR5 antibody, FHFR antibody, FGFRL1 antibody, FGFR-like protein antibody, FGF homologous factor receptor antibody, FGFR-5 antibody, FGF receptor-like protein 1 antibody, fibroblast growth factor receptor-like 1 antibody, fibroblast growth factor receptor 5 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
PBS
Calculated Molecular Weight
55 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 150 and 476 (Q8N441) of FGFR5
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
FGFR-5 antibody detects FGFR-5 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FGFR-5 protein stained by FGFR-5 antibody (107-10404) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FGFR-5 protein stained by FGFR-5 antibody (107-10404) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
FGFR-5 antibody detects FGFR-5 protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
D. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
FGFR-5 antibody (107-10404) dilution: 1:1000
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
D. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
FGFR-5 antibody (107-10404) dilution: 1:1000
FGFR-5 antibody detects FGFR-5 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
FGFR-5 antibody (107-10404) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
FGFR-5 antibody (107-10404) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Oral CA Cal27 xenograft, using FGFRL1(107-10404) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.