Filamin B antibody [N1], N-Terminus, 10 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2317 |
| Protein Name / Synonyms | Filamin B, N-term |
| Concentration (lot specific) | 10 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ABP-278 antibody, ABP-280 antibody, AOI antibody, DKFZp686A1668 antibody, DKFZp686O033 antibody, FH1 antibody, FLN1L antibody, LRS1 antibody, SCT antibody, TABP antibody, TAP antibody, FLNB antibody, thyroid autoantigen antibody, ABP-280 homolog antibody, filamin-3 antibody, filamin homolog 1 antibody, actin-binding-like protein antibody, Larsen syndrome 1 (autosomal dominant) antibody, actin binding protein 278 antibody, filamin-B antibody, beta-filamin antibody, filamin B, beta antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
278 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 30 and 269 of Filamin B (Uniprot ID# O75369)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B(107-10249) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
Filamin B antibody [N1], N-term detects FLNB protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Filamin B antibody [N1], N-term (107-10249) dilution: 1:500.
Sample: Paraffin-embedded U87 xenograft.
Filamin B antibody [N1], N-term (107-10249) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
107-10249 diluted at 1:1000
A: U87-MG
5% SDS PAGE
107-10249 diluted at 1:1000
Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell extract
5 % SDS-PAGE
Filamin B antibody [N1], N-term (107-10249) dilution: 1:1000
A. 30 µg NIH-3T3 whole cell extract
5 % SDS-PAGE
Filamin B antibody [N1], N-term (107-10249) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.