Flightless I antibody [N3C1], Internal, 0.88 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2314 |
| Protein Name / Synonyms | Flightless I, Internal |
| Concentration (lot specific) | 0.88 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLI antibody, FLIL antibody, Fli1 antibody, MGC39265 antibody, FLII antibody, protein flightless-1 homolog antibody, flightless I homolog (Drosophila) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
145 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I (Uniprot ID# Q13045)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(107-10598) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I(107-10598) antibody at 1:200 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10598 diluted at 1:1000
A: Mouse brain
5% SDS PAGE
107-10598 diluted at 1:1000
Sample(30 ug whole cell lysate)
A:Raji
5% SDS PAGE
107-10598 diluted at 1:1000
A:Raji
5% SDS PAGE
107-10598 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.