Synonyms
MGC21624 antibody, FLRT1 antibody, leucine-rich repeat transmembrane protein FLRT1 antibody, fibronectin-like domain-containing leucine-rich transmembrane protein 1 antibody, fibronectin leucine rich transmembrane protein 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
71 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 35 and 255 of FLRT1 (Uniprot ID# Q9NZU1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using FLRT1(107-10536) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCA, using FLRT1(107-10536) antibody at 1:100 dilution.

Sample (30µg whole cell lysate)
A: H1299
B: HeLa S3
7.5% SDS PAGE
107-10536 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.