Rabbit Anti-Human Fructose 6 Phosphate Kinase (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08237 |
| Gene Id | 5213 |
| Gene Symbols | PFKM |
| Protein Name / Synonyms | Fructose 6 Phosphate Kinase (N-term) |
| Clonality | Polyclonal |
| Preparation | This Fructose 6 Phosphate Kinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the N-terminal region of human Fructose 6 Phosphate Kinase. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Phosphorylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Specificity
Other Names
PFKM; PFKX; 6-phosphofructokinase, muscle type; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; Phosphohexokinase
NCBI Accession #
NP_000280.1;NP_001160158.1;NP_001160159.1;NP_001160160.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
85183
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of lysate from human skeletal muscle tissue lysate, using PFKM Antibody ?S137?(Cat# 102-17817). 102-17817 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.