Synonyms
RP11-127F21 antibody, SPRYD1 antibody, FSD2 antibody, SPRY domain-containing protein 1 antibody, fibronectin type III and SPRY domain-containing protein 2 antibody, SPRY domain containing 1 antibody, fibronectin type III and SPRY domain containing 2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
85 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 215 of FSD2 (Uniprot ID# A1L4K1)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SAOS-2 xenograft, using FSD2(107-10647) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10647 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.