Rabbit Anti-Human GABARAP (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95166 |
| Gene Id | 11337 |
| Gene Symbols | GABARAP |
| Protein Name / Synonyms | GABARAP (N-term) |
| Clonality | Polyclonal |
| Preparation | This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, IHC-P, Western Blotting |
| Research Area | Neuroscience, Autophagy, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Rabbit
Other Names
GABARAP; FLC3B; Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein; MM46
NCBI Accession #
NP_009209.1
Other Accession #
P60517; Q8MK68; Q9DCD6; Q9GJW7
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
13918
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50
 | Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13733 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. |
 | Western blot analysis of GABARAP (arrow) using purified Pab (Cat# 102-13733). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies). |
 | Rabbit anti-GABARAP RayBiotech 102-13733 1:1000 dilution in 5% scimmed milk powder. Secondary antibody goat anti-rabbit-HRP ECL detection 30 sec on X-ray film. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP Antibody (N-term)(Cat# 102-13733), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | GABARAP antibody was tested in mouse MEFs and mouse motor neurons. Cells were fixed in 3% PFA and permeabilized with 100% methanol. Antibodies used were RayBiotech anti-GABARAP (1:500, labeled with anti-rabbit 488 Alexa) and Covance anti-Tuj1 (1:1000, labeled with anti-mouse 647). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.