Rabbit Anti-Human GADD45A (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P24522 |
| Gene Id | 1647 |
| Gene Symbols | GADD45A |
| Protein Name / Synonyms | GADD45A (C-term) |
| Clonality | Polyclonal |
| Preparation | This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Hamster
Other Names
GADD45A; DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein
NCBI Accession #
NP_001186670.1;NP_001186671.1;NP_001915.1
Other Accession #
P48317; P48316; P24523; Q3ZBN6
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
18336
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of GADD45A Antibody (C-term) (Cat# 102-15140) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab. |
 | GADD45A Antibody (C-term) (Cat# 102-15140) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-term) (Cat# 102-15140). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.