GALNS antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2588 |
| Protein Name / Synonyms | GALNS |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ17434 antibody, FLJ42844 antibody, FLJ98217 antibody, GALNAC6S antibody, GAS antibody, MPS4A antibody, GALNS antibody, galactose-6-sulfate sulfatase antibody, chondroitinase antibody, N-acetylgalactosamine-6-sulfate sulfatase antibody, galNAc6S sulfatase antibody, chondroitinsulfatase antibody, N-acetylgalactosamine-6-sulfatase antibody, galactosamine (N-acetyl)-6-sulfate sulfatase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
58 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 20 and 259 of GALNS
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS(107-10906) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.
GALNS antibody detects GALNS protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:1000
GALNS antibody detects GALNS protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:500
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
GALNS antibody (107-10906) dilution: 1:500
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS(107-10906) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10906 diluted at 1:1000
A: H1299
7.5% SDS PAGE
107-10906 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.