GALNT7 antibody [N1C2], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 51809 |
| Protein Name / Synonyms | GALNT7 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
GALNAC-T7 antibody, GalNAcT7 antibody, GALNT7 antibody, pp-GaNTase 7 antibody, N-acetylgalactosaminyltransferase 7 antibody, polypeptide GalNAc transferase 7 antibody, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 antibody, polypeptide N-acetylgalactosaminyltransferase 7 antibody, protein-UDP acetylgalactosaminyltransferase 7 antibody, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
75 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 24 and 515 of GALNT7 (Uniprot ID# Q86SF2)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
GALNT7 antibody [N1C2] detects GALNT7 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (107-10582) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GALNT7 protein stained by GALNT7 antibody [N1C2] (107-10582) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded Endometrial CA, using GALNT7(107-10582) antibody at 1:100 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10582 diluted at 1:5000
A: Mouse brain
7.5% SDS PAGE
107-10582 diluted at 1:5000
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10582 diluted at 1:10000
A: A431
7.5% SDS PAGE
107-10582 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.