Synonyms
ACT antibody, ACTG antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, cytoskeletal gamma-actin antibody, "actin, cytoplasmic 2 antibody", "actin, gamma 1 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
42 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 375 of gamma Actin
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Spleen, using gamma Actin(107-10289) antibody(10 µg/ml).

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
107-10289 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10289 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.