Rabbit Anti-Human GAPDH (C-term R248) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04406 |
| Gene Id | 2597 |
| Gene Symbols | GAPDH |
| Protein Name / Synonyms | GAPDH (C-term R248) |
| Clonality | Polyclonal |
| Preparation | This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken, Pig
Other Names
GAPDH; GAPD; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
NCBI Accession #
NP_001243728.1;NP_002037.2
Other Accession #
P04797; P00355; P16858; P00356
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
36053
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (C-term R248)(Cat# 102-17673).102-17673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. |
 | Western blot analysis of GAPDH Antibody (C-term R248) (Cat# 102-17673) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HepG2 cells using GAPDH Antibody (C-term R248)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248)(Cat# 102-17673) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
 | Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248)(Cat# 102-17673) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.